Chloroplast division in cultured cells of the hornwortAnthoceros punctatus |
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Authors: | Yoshihiro Izumi Kanji Ono Masayuki Takamiya Kiichi Fukui |
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Institution: | (1) Department of Biological Science, Faculty of Science, Kumamoto University, 860 Kumamoto, Japan;(2) Hokuriku National Agricultural Experiment Station, 943-01 Joetsu, Japan |
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Abstract: | Using cultured cells of the hornwortAnthoceros punctatus, the change in the relative chloroplast DNA content in each stage of chloroplast division was investigated to clarify the
relationship between the division cycle of a chloroplast and a cell nucleus. Samples of cultured cells were stained with 4′,6-diamidino-2-phenylindole
(DAPI) and then observed with an epifluorescence microscope and a chromosome image analyzing system (CHIAS). A chloropiast
in cultured cells duplicated DNA with an increase in size. When a chloroplast began to divide, it was constricted in the middle,
taking a dumbbell shape, and then divided into two daughter chloroplasts. In cultured cells of this species, the pattern of
quantitative change of chloroplast DNA, that is, the DNA replication pattern of chloroplasts, corresponded to that of cell
nuclear DNA in mitosis. |
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Keywords: | Anthoceros punctatus Bryophyte Chloroplast division DNA content Hornwort |
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