Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus
officinalis (Lamiaceae), using enriched genomic libraries |
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Authors: | J G Segarra-Moragues G Gleiser |
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Institution: | (1) Centro de Investigaciones sobre Desertificación (CIDE-CSIC-UV-GV), Camí de la Marjal s/n, 46470 Albal, Valencia, Spain |
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Abstract: | An enrichment protocol was used to isolate and characterise microsatellite loci in Rosmarinus officinalis, a Mediterranean chamephyte. Twelve microsatellite loci were characterised and amplified a total of 117 alleles in a sample
of 30 individuals from one population, with an average of 9.75 alleles per locus. Observed heterozygosities ranged from 0.333
to 0.900. Cross-species transferability was also assayed in the two other species of the genus. The cumulated probabilities
of exclusion for paternity and parentage of the 12 loci were of 0.999971 and 1, respectively, supporting the usefulness of
these microsatellite loci for parentage analyses. Nine out of 12 microsatellite loci amplified in the two species and were
polymorphic detecting a total of 49 and 45 in R. eriocalyx and R. tomentosus, respectively. Twenty-two alleles were exclusive of R. eriocalyx and 12 of R. tomentosus, additionally, three alleles were shared between these two species but were otherwise absent in the analysed individuals
of R. officinalis. In total, this set of markers amplified 154 different microsatellite alleles, supporting their usefulness to conduct population
genetic, reproductive biology and hybridisation studies in Rosmarinus. |
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Keywords: | Labiatae Rosmarinus Genetic diversity SSR |
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