Kinetics of the enzymatic hydrolysis of cellulose: 2. A mathematical model for the process in a plug-flow column reactor |
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Authors: | A.V. Gusakov A.P. Sinitsyn A.A. Klyosov |
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Affiliation: | Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 117234, USSR;A. N. Bach Institute of Biochemistry, 33 Leninsky Pr., Moscow 117071, USSR |
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Abstract: | The kinetics of enzymatic cellulose hydrolysis in a plug-flow column reactor catalysed by cellulases [see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4] from Trichoderma longibrachiatum adsorbed on cellulose surface have been studied. The maximum substrate conversion achieved was 90–94%. The possibility of enzyme recovery for a reactor of this type is discussed. A mathematical model for enzymatic cellulose hydrolysis in a plug-flow column reactor has been developed. The model allows for the component composition of the cellulase complex, adsorption of cellulases on the substrate surface, inhibition by reaction products, changes in cellulose reactivity and the inactivation of enzymes in the course of hydrolysis. The model affords a reliable prediction of the kinetics of d-glucose and cellobiose formation from cellulose in a column reactor as well as the degree of substrate conversion and reactor productivity with various amounts of adsorbed enzymes and at various flow rates. |
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Keywords: | Cellulose cellulases hydrolysis cellobiose kinetics modelling plug-flow column reactor |
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