Production of nisin Z using <Emphasis Type="Italic">Lactococcus lactis</Emphasis> IO-1 from hydrolyzed sago starch |
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Authors: | Octavio Carvajal-Zarrabal Cirilo Nolasco-Hipólito Kopli B Bujang Ayaaki Ishizaki |
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Institution: | 1.Instituto de Medicina Forense,Universidad Veracruzana,Boca del Río,Mexico;2.NECFER Corporation, F-BIC101,Kurume-Shi, Fukuoka,Japan;3.Deptartment of Molecular Biology,Faculty of Resource Science and Technology, Universiti Malaysia Sarawak,Kota Samarahan,Malaysia;4.R&D Division F-BIC101,NECFER CORPORATION,Kurume-Shi, Fukuoka,Japan |
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Abstract: | A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of
nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity
of nisin Z, as 50,000 IU l−1 h−1 using a cell concentration of 15 g l−1, 30°C, pH 5.5 and a dilution rate of 1.24 h−1. Adding 10 g l−1 YE and 2 g l−1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed
nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase
mode with high metabolic activity. |
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Keywords: | Nisin Z Bacteriocin Sago starch Membrane bioreactor Lactococcus lactis IO-1 |
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