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Tenascins are associated with lipid rafts isolated from mouse brain
Authors:Kappler Joachim  Baader Stephan L  Franken Sebastian  Pesheva Penka  Schilling Karl  Rauch Uwe  Gieselmann Volkmar
Affiliation:Physiologisch-Chemisches Institut, Nussallee 11, 53115 Bonn, Germany. kappler@institut.physiochem.uni-bonn.de
Abstract:
Lipid rafts are microdomains of the plasma membrane which are enriched in glycosphingolipids and specific proteins. The reported interactions of several raft-associated proteins (such as, e.g., F3) with tenascin C and tenascin R prompted us to consider that these oligomeric multidomain glycoproteins of the extracellular matrix (ECM) could associate with rafts. Here, we show punctate immunocytochemical distributions of tenascin C (TN-C) and tenascin R (TN-R) at the membrane surface of neural cells resembling the pattern reported for raft-associated proteins. Moreover, cholesterol depletion with methyl-beta-cyclodextrin reduced the punctate surface staining of TN-C. Consistently, TN-C was associated with lipid rafts of neonatal mouse brain according to sucrose density gradient centrifugation experiments. Furthermore, TN-R was also found in rafts prepared from myelin of adult mice. Thus, brain-derived tenascins are able to associate with lipid rafts.
Keywords:Tenascin   Lipid rafts   Cerebellum
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