Cloning of a bovine orphan transporter and its short splicing variant |
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| Authors: | Sakata K Shimada S Yamashita T Inoue K Tohyama M |
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| Affiliation: | Department of Anatomy and Neuroscience, Osaka University Medical School, Suita, Japan. sakata@anat2.med.osaka-u.ac.jp |
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| Abstract: | We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining. |
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