| 用生物素-中性抗生物素蛋白结合导致的位阻来控制连接生物素的缓激肽生物活性(英) |
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| 引用本文： | 张海黔,篠原宽明,顾宁,佐々木裕司,宍戸昌彦.用生物素-中性抗生物素蛋白结合导致的位阻来控制连接生物素的缓激肽生物活性(英)[J].生物化学与生物物理进展,2002,29(1):60-63. |
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| 作者姓名： | 张海黔 篠原宽明 顾宁 佐々木裕司 宍戸昌彦 |
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| 作者单位： | 1. 东南大学吴建雄研究室 南京210096 中国
2. 冈山大学生物科学与技术系 冈山700-8530 日本 |
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| 摘 要： | 缓激肽是一含有9个氨基酸残基的多肽，其残基序列为Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9-OH，在激肽释放酶的作用下， 从其大的前体多肽——激肽原而形成的.许多发病机理，如发炎、疼痛、哮喘等都与缓激肽有关. 它能与PC12细胞表面的受体作用，引起细胞器内的钙离子释放，在共焦显微镜下，通过观察钙指示剂Fluo-3荧光增加来监测缓激肽的生物活性.在这项研究中，利用固相肽合成方法合成了连接生物素的缓激肽，Biotin-Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9-OH，通过对其生物活性的研究发现：a.它能保持象天然的缓激肽那样的生物活性；b.由于中性抗生物素蛋白与连接的生物素的结合引起的空间位阻阻碍它与细胞表面受体的相互作用，从而抑制了它的生物活性；c.在有自由的生物素存在的条件下，自由生物素与连接生物素与中性抗生物素蛋白的竞争结合，能够使得与中性抗生物素蛋白结合的连接生物素的缓激肽从抗生物素蛋白上脱离，因而恢复其生物活性.因此，可利用生物素和抗生物素蛋白来控制连接生物素的缓激肽的生物活性.这对于研究生物体系中生物活性的结构相关性具有重要的意义.
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| 关 键 词： | 缓激肽，空间位阻，生物活性，荧光，生物素，中性抗生物素蛋白 |
| 收稿时间： | 2001/4/19 0:00:00 |
| 修稿时间： | 2001/5/24 0:00:00 |
Bound Biotin-neutravidin Inducing Steric Hindrance Used for Controlling Bioactivity of Bradykinin Linked with Biotin |
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| ZHANG Hai-Qian,S.HIROAKI,GU Ning,S.HIROSHI and S.MASAHIKO.Bound Biotin-neutravidin Inducing Steric Hindrance Used for Controlling Bioactivity of Bradykinin Linked with Biotin[J].Progress In Biochemistry and Biophysics,2002,29(1):60-63. |
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| Authors: | ZHANG Hai-Qian SHIROAKI GU Ning SHIROSHI and SMASAHIKO |
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| Institution: | Laboratory of Wu Chien-shiung, Southeast University, Nanjing 210096, China;Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1, Tsushima-naka, Okayama 700-8530, Japan;Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1, Tsushima-naka, Okayama 700-8530, Japan;Laboratory of Wu Chien-shiung, Southeast University, Nanjing 210096, China;Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1, Tsushima-naka, Okayama 700-8530, Japan;Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1, Tsushima-naka, Okayama 700-8530, Japan |
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| Abstract: | Biotin-linked bradykinin was synthesized by solid phase peptide synthesis for development of a functionalized peptide and study on structure-relevant bioactivity in biological system. In PC12 cell system, bioactivity of the synthetic peptide was evaluated and found to be controllable in the presence of neutravidin and free biotin. The controlling mechanism had been discussed and could be ascribed to steric hindrance induced by binding of neutravidin to the linked biotin. Moreover, influence of competitive binding between the free biotin and the linked biotin to the neutravidin had also investigated into and could be employed for switching the bioactivity on and off. |
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| Keywords: | bradykinin steric hindrance bioactivity fluorescence biotin neutravidin |
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