Efficient plant regeneration from Indica (group 1) rice protoplasts of one advanced breeding line and three varieties |
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| Authors: | Shiping Zhang |
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| Affiliation: | (1) Tissue Culture Laboratory, The International Rice Research Institute, P.O. Box 933, 1099 Manila, Philippines;(2) Present address: Division of Plant Biology, MRC 7, ILTAB, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037 La Jolla, CA, USA |
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| Abstract: | ![]()
Regenerable embryogenic suspensions were established from one Indica (group 1) rice advanced breeding line and 9 Indica (group 1) rice varieties in 6–8 weeks. Four were chosen for protoplast culture and plant regeneration. About 4–7×107 protoplasts were isolated from one gram of 8-week-old cell suspension. High plating efficiency (30.5%) and colony formation (13.7%) were obtained using nurse culture methods. A high plant regeneration frequency (67.5%) was observed for line IR57311-95-2-3. In total, 322 plants were regenerated. All the regenerated plants were fertile.Abbreviation 2,4-D 2,4-dichlorophenoxy acetic acid - NAA 1-naphtalene acetic acid |
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| Keywords: | Indica rice-Plant regeneration-Protoplasts |
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