Ultrahigh diterpenoid tanshinone production through repeated osmotic stress and elicitor stimulation in fed-batch culture of <Emphasis Type="Italic">Salvia miltiorrhiza</Emphasis> hairy roots |
| |
Authors: | Jian-Yong Wu Ming Shi |
| |
Institution: | (1) Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong |
| |
Abstract: | Hyperosmotic stress (OS, created with 50 g/L sorbitol) and a yeast elicitor (YE, polysaccharide fraction of yeast extract)
applied to Salvia miltiorrhiza hairy root cultures had a synergistic effect on the diterpenoid tanshinone production. With a single OS+YE treatment and
nutrient feeding, the total tanshinone content of roots was increased by sevenfold (from 0.2 to 1.6 mg/g dry weight (dw))
and the volumetric yield by 13-fold (from 1.95 to 27.4 mg/L) compared to the batch control culture. With repeated feeding
of OS and nutrient medium in an extended fed-batch culture process (i.e., 10 mL fresh medium with 50 g/L sorbitol 25 mg/L
YE, every 5 days from day 21 to day 60), the total tanshinone content of roots was increased to 18.1 mg/g dw (or 1.8 wt.%)
and the volumetric tanshinone yield to 145 mg/L, which were about 100-fold and 70-fold of those, respectively, in the batch
control. Another interesting finding was the presence of root fragments (fine particles) with extremely high tanshinone content
in the OS+YE treated cultures. It was also possible to reuse the sorbitol medium for the hairy root growth and tanshinone
production to reduce the medium expenses. |
| |
Keywords: | Salvia miltiorrhiza Hairy root Tanshinones Osmotic stress Yeast elicitor Fed-batch culture |
本文献已被 PubMed SpringerLink 等数据库收录! |
|