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| Transient Decrease of Light-harvesting Complex Ⅱ Phosphorylation Level by Hypoosmotic Shock in Dark-adapted Dunaliella salina | |
| 引用本文： | Liu XD,Hu FH,Shen YG. Transient Decrease of Light-harvesting Complex Ⅱ Phosphorylation Level by Hypoosmotic Shock in Dark-adapted Dunaliella salina[J]. Acta biochimica et biophysica Sinica, 2006, 38(2): 104-109 |
| 作者姓名： | Liu XD Hu FH Shen YG |
| 作者单位： | Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200032, China |
| 摘 要： | This study investigated the regulation of major light harvesting chlorophyll a/b protein （LHCⅡ） phosphorylation by hypoosmotic shock in dark-adapted Dunaliella salina cells. When the external NaCI concentration decreased in darkness, D. salina LHCⅡ phosphorylation levels transiently dropped within 20 min and then restored gradually to basal levels. The transient decrease in LHCII phosphorylation levels was insensitive to NaF, a phosphatase inhibitor. Inhibition of intracellular ATP production by addition of an uncoupler or an ATP synthase inhibitor increased LHCⅡ phosphorylation levels in D. salina cells exposed to hypoosmotic shock. Taken together, these results indicate that hypoosmotic shock inhibits the LHCⅡ phosphorylation process. The related mechanism and physiological significance are discussed. |
| 关 键 词： | 磷酸化作用 杜氏藻 盐碱滩 叶绿素 |
| 收稿时间： | 2005-09-14 |
| 修稿时间： | 2005-09-142005-12-08 |
Transient decrease of light-harvesting complex II phosphorylation level by hypoosmotic shock in dark-adapted Dunaliella salina |
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| Liu Xian-De,Hu Fen-Hong,Shen Yun-Gang. Transient decrease of light-harvesting complex II phosphorylation level by hypoosmotic shock in dark-adapted Dunaliella salina[J]. Acta biochimica et biophysica Sinica, 2006, 38(2): 104-109 | |
| Authors: | Liu Xian-De Hu Fen-Hong Shen Yun-Gang |
| Affiliation: | Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200032, China. |
| Abstract: | This study investigated the regulation of major light harvesting chlorophyll a/b protein (LHCII) phosphorylation by hypoosmotic shock in dark-adapted Dunaliella salina cells. When the external NaCl concentration decreased in darkness, D. salina LHCII phosphorylation levels transiently dropped within 20 min and then restored gradually to basal levels. The transient decrease in LHCII phosphorylation levels was insensitive to NaF, a phosphatase inhibitor. Inhibition of intracellular ATP production by addition of an uncoupler or an ATP synthase inhibitor increased LHCII phosphorylation levels in D. salina cells exposed to hypoosmotic shock. Taken together, these results indicate that hypoosmotic shock inhibits the LHCII phosphorylation process. The related mechanism and physiological significance are discussed. |
| Keywords: | ATP content Dunaliella salina hypoosmotic shock light harvesting chlorophyll a/b protein (LHCII) phosphorylation |
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