甲基磺酸乙酯(EMS)对水稻离体幼穗影响的初步研究

用不同浓度的EMS处理水稻离体幼穗,测定培养初期的呼吸强度、细胞色素氧化酶活性和同工酶以及苹果酸脱氢酶同工酶,发现低浓度的EMS对细胞呼吸强度以及细胞色素氧化酶活性有刺激作用,而高浓度的EMS则表现为抑制效应。呼吸强度和酶活性与幼穗培养力之间具有平行关系。研究结果表明,EMS的原初生理效应是影响幼穗细胞的代谢过程,幼穗体细胞诱导频率的改变是幼穗培养初期供能系统受到影响的结果。

PRELIMINARY STUDY ON THE EFFECT OF EMS UPON THE YOUNG PANICLES OF RICE IN VITRO

Young panicles of rice (Oryza sativa L.) were treated with a range of concentration of EMS for 3 hours before cultured in vitro. The respiratory intensity of young panicles was determined on the second, fourth, sixth day respectively, and the cytochrome oxidase activity and isoenzymes of cytochrome oxidase and malate dehyrogenase were analysed on the forth day. Tha experimental results show that the effects of EMS on respiratory intensity and activities of two enzymes of young panicles are in accordance with the effects of the mutagen on de-and re-differentiation of the young panicle cells. EMS affects metabolic processes in the cells at first and the induction rate of the young panicle callus changes consequently.