| 应用分光光度法、薄层扫描及高效液相色谱法进行药根碱的定量分析 |
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| 引用本文： | 卢大炎；冯明鸿；李新明；侯嵩生.应用分光光度法、薄层扫描及高效液相色谱法进行药根碱的定量分析[J].武汉植物学研究,1989,7(4):363-367. |
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| 作者姓名： | 卢大炎；冯明鸿；李新明；侯嵩生 |
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| 作者单位： | 中国科学院武汉植物研究所
(卢大炎，冯明鸿，李新明)，中国科学院武汉植物研究所(侯嵩生) |
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| 摘 要： | 本工作比较考察了用分光光度法、薄屋扫描和高效液相色谱定量测定药根碱(Jatrorrhizine)的三种分析方法。对于九连小檗(Berberis julianae Schneid)细胞培养物的抽提液,其药根碱的含量可直接用分光光度法在波长430nm处进行测定,也可经薄层层析后用450nm波长进行反射吸收扫描定量,还可用高效液相色谱ODS反相柱经磷酸-乙腈体系洗脱,用346nm波长进行检测。三种方法均可得到良好的分析结果。
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| 关 键 词： | 药根碱 分光光度法 薄层扫描法 高效液相色谱法 九连小檗 |
QUANTITATIVE ANALYSIS OF JATRORRHIZINE BY SPECTROPHOTOMETRY, THIN LAYER CHROMATOGRAPHIC SCANNING AND HIGH PERFORMANCE LIQUID CHROMATOGRPHY |
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| Lu Dayan,Feng Minghong,Li Xinming,Hou Songsheng.QUANTITATIVE ANALYSIS OF JATRORRHIZINE BY SPECTROPHOTOMETRY, THIN LAYER CHROMATOGRAPHIC SCANNING AND HIGH PERFORMANCE LIQUID CHROMATOGRPHY[J].Journal of Wuhan Botanical Research,1989,7(4):363-367. |
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| Authors: | Lu Dayan Feng Minghong Li Xinming Hou Songsheng |
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| Institution: | Wuhan Institute of Botany, Academia Sinica |
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| Abstract: | In this paper, three methods for determination of jatrorrhizine are reported and compared with each other by spectrophotometry, TLC-scanning and RP-HPLC. For the Berberis julianae Schneid cell cultural extracts, jatrorrhizine can be determined directly by spectrophotometry at wavelength 430 nm. The sample can also be determined by reflective absorption scanning at wavelength 450nm after developed on TLC plate, or it can be injected into HPLC on ODS revers phase column and adopted phosphoric acid-acetonitrile system as mobile phase, detection at 346nm. The excellect results obtained with any one of these methods were in good accord with each other. |
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| Keywords: | Jatrorrhizine Spectrophotometry TLC-scanning HPLC Berberis julianae Schneid |
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