Studies on phytoremediation potentiality of Typhonium flagelliforme for the degradation of Brilliant Blue R |
| |
Authors: | Anuradha N Kagalkar Umesh B Jagtap Jyoti P Jadhav Sanjay P Govindwar Vishwas A Bapat |
| |
Institution: | (1) Department of Biochemistry, Shivaji University, Kolhapur, 416004, India;(2) Department of Biotechnology, Shivaji University, Kolhapur, 416004, India; |
| |
Abstract: | In vitro culture plants of Typhonium flagelliforme were found to decolorize a variety of dyes, including Malachite Green, Red HE 8B, Methyl Orange, Reactive Red 2, Direct Red
5B (DR5B), Red HE 7B, Golden Yellow HER, Patent Blue, and Brilliant Blue R (BBR), to varying extents within 4 days. The enzymatic
analysis of plant roots of aseptically raised plantlets performed before and after degradation of the dye BBR by these plantlets
showed a significant induction in the activities of peroxidase, laccase, tyrosinase, and 2,6-dichlorophenol-indophenol reductase,
which indicated the involvement of these enzymes in the metabolism of the dye. Comparative study of the enzyme status of the
plants Typhonium flagelliforme and Blumea malcolmii during the degradation of DR5B and BBR showed marked variations in the enzyme profile with respect to the use of different
sources of the enzyme. Phytoremediation of BBR using Typhonium flagelliforme was confirmed with high performance liquid chromatography and Fourier transform infrared spectroscopy analysis performed
before and after the degradation of the dye. One of the products of the metabolism of the dye was identified as 4-(4-ethylimino-cyclohexa-2,5-dienylidinemethyl)-phenylamine
with the aid of gas chromatography–mass spectroscopy (GC–MS) analysis. Significant decrease in the American Dye Manufacturer’s
Institute, biological oxygen demand, and chemical oxygen demand values of synthetic mixture of textile dyes and industrial
effluent confirmed the decolorization and detoxification. Phytotoxicity studies also revealed the nontoxic nature of the metabolites
of BBR. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|