Old phage, new insights: Two recently recognized mechanisms of transcriptional regulation in bacteriophage T4 development |
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| Authors: | E.N Brody G.A Kassavetis M Ouhammouch G.M Sanders R.L Tinker E.P Geiduschek |
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| Affiliation: | Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260-1300, USA; Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0634, USA |
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| Abstract: | Abstract The regulation of bacteriophage T4 middle and late gene expression involves previously unrecognized mechanisms. Middle transcription requires a DNA-binding transcriptional activator and a σ 70-binding co-activator. The coupling of late transcription to DNA replication is effected by a DNA-tracking protein that is loaded onto DNA by an assembly factor at enhancer-like entry sites. Late transcription also requires an RNA polymerase core-binding co-activator. The co-activators of T4 middle and late transcription share the property of depressing unactivated, basal transcription. |
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| Keywords: | Transcriptional regulation Bacteriophage T4 DNA-tracking proteins Anti-sigma Replication-transcription coupling Co-activators |
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