经大豆DNA溶液处理的水稻后代种子的粗蛋白和氨基酸含量分析初报

以大豆为外源DNA供体,用浸种及幼苗期浇灌法和花粉管通道法直接将大豆总DNA导入受体水稻,经常规栽培获得水稻后代种子。采用微量凯氏定氮法和氨基酸自动分析仪进行水稻后代种子糙米的粗蛋白含量和氨基酸含量的测定。结果显示:三组经大豆DNA溶液处理获得的水稻后代种子糙米粗蛋白平均含量分别为16.42%、16.80%和19.87%,与对照组相比有明显提高,统计分析都达到极显著的差异(P < 0.01);在氨基酸含量测定中,有些材料的总氨基酸(除色氨酸以外)含量高达17.20%、16.86%和16.09%,其中赖氨酸的含量分别为0.60%、0.60%和0.57%,与对照组相比也有明显提高,统计分析差异也都达到极显著水平(P < 0.01)。本试验结果充分说明,利用大豆总DNA的导入方法有可能达到迅速有效地提高稻米蛋白质及赖氨酸含量的目的。

THE CONTENT ANALYSES OF CRUDE PROTEIN, AMINO ACID IN RICE SEEDS INDUCED BY EXOGENOUS DNA FROM SOYBEAN

DNA was extracted and purified from soybean seeds. The exogenous DNA from soybean was introduced into rice seeds by soaking seeds, moreover irrigating seedlings, and through the pollen-tube pathway after self-pollination. The contents of crude protein and amino acid in the offspring's seeds were analyzed. The results indicated: (1).The average contents of crude protein in three groups of the offspring's seeds induced by exogenous DNA from soybean were 16.42%、16.80% and 19.87%(Table 1), increasing distinctly over their contrasts.(2). All of the contents of amino acid (except try) in some offspring's seeds induced by exogenous DNA from soybean were 17.20%、16.86% and 16.09%(Table 2), greatly increasing over the contrasts. The lysine contents in the three offspring's seeds were respectively 0.60%、0.60% and 0.57% (Table 2).