阻断子宫动脉建立FGR大鼠模型的研究

目的通过暂时阻断妊娠期大鼠子宫血供的方法建立子宫缺血引起胎儿生长受限的动物模型。方法根据大鼠子宫动脉是卵巢动脉的一个分支的解剖特点,于孕鼠妊娠第15天时施行手术暂时阻断卵巢动脉并于第21天行剖宫产术,术后称量新生胎仔体重及胎盘、脑、心、肝、肺、肾等重要脏器重量,对比各组间新生胎仔的预后的不同,并对照研究阻断血供10、20、30及40 min对胎仔的不同影响。结果妊娠晚期阻断孕鼠卵巢动脉20min可成功构建胎儿生长受限模型,这种方法与阻断动脉血流30或40 min相比,手术时间短,技术要求不高,胎仔死亡率与对照组差异无显著性(P>0.05)。各实验组较对照组新生胎仔体重及胎盘、各重要脏器重量均明显降低(P<0.05)。结论通过阻断卵巢动脉从而阻断子宫动脉血流,成功建立缺血缺氧性FGR孕鼠模型。该模型重复性好,操作简便,并可成功设立同体对照,为进行FGR相关的产科理论研究提供了一个有利的技术平台。

Establishment Of a Rat Model of Fetal Growth Restriction by Interruption Of the Uterine Artery B1ood Flow

Objective To establish an experimental animal model of fetal growth restriction(FGR) by temporary interruption of the uterine artery blood flow during late stage of pregnancy,and provide a useful animal model to investigate the FGR mechanism induced by hypoxia.Methods Based on the fact that the rat uterine artery is a branch of ovary artery,the pregnant rats undergone an operation temporarily interrupting the ovary artery blood flow at 15th day of pregnancy and caesarean birth at 21~(st) day of pregnancy.After the caesarean birth,the body weight of fetus,weight of placenta,weight of the brain,heart,liver,lungs and kidneys of the fetus in every group were compared.The prognosis of each group and the effect of different time of artery blood interruption(10 min,20 min,30 min and 40 min) were compared and analyzed.Results The rat FGR model was successfully established by interrupting the ovary artery in pregnant rats for 20 minutes.This method was simple to perform and needs less time than the operation interrupting the artery blood for 30 or 40 minutes,while the fetal mortality was similar between experimental group and control group (P>0.05).In the rat model,the weights of new-laid rats and their placenta,brain,heart,liver,lungs and kidneys were all decreased in comparison with those in control group(P<0.05).Conclusion By temporarily clamping the ovary artery blood flow in pregnant rats,an animal FGR model has been successfully established in rats.The model is simple to perform and well reproducible,with autogenous control,and provides a useful approach in FGR research.