陇南越夏区自生麦苗条形柄锈菌毒性表型及分子基因型分析

为了解陇南地区越夏自生麦苗上条形柄锈菌的毒性组成及群体遗传结构，将采自天水、陇南及定西的自生麦苗上的39个单孢子堆菌系采用中、美鉴别寄主毒性分析法和TP-M13-SSR荧光标记技术，进行毒性鉴定并对其基因组DNA进行SSR标记分析。结果显示：在中国鉴别寄主上供试菌系被区分为9个致病类型，在美国鉴别寄主上则得到24个毒性类型，在中美鉴别寄主上共得到30个毒性表型。通过中国鉴别寄主鉴定的CYR32、CYR33为优势小种，毒性比率分别达到35.9%和30.8%。SSR标记将这些菌系划分为36个基因型，毒性分析与

The virulence phenotype and molecular genotype analysis of stripe rust on volunteer wheat seedlings in South Gansu

Thirty-nine single pustule isolates were collected from volunteer wheat seedlings in Tianshui, Longnan and Dingxi, southern Gansu Province, for exploring the virulent component and population structure of Puccinia striiformis f. sp. tritici on volunteers by virulence analysis using both Chinese and U.S. differentials and TP-M13-SSR fluorescent labeling methods. From the isolates, 9 races were identified based on reactions on the 19 Chinese differentials and 24 races on the 20 U.S. ones. A total of 30 virulence patterns were identified among the 39 isolates based on their reactions on both Chinese and U.S. differentials. Based on Chinese differentials, CYR32 and CYR33 were the dominant races, the virulence frequencies were 35.9% and 30.8%, respectively. The 39 isolates were clustered into 6 groups by both the virulence and the SSR data, which were switched to the middle value of the similarity range data. Virulence and SSR data had a low (r=0.043) correlation. There were same phenotypes or genotypes (similarity 100%) from different geographical populations by both virulence and SSR analyses, and the gene flow value (Nm) was 2.59. The results above reveal that there exists abundant virulence phenotype and molecular genotype diversity of Pst on Longnan volunteer wheat, and there is frequent gene flow in different populations, which are reserved high diversity source pathogens for autumn seedlings in Longnan over-summering zone. The results of present study can provide a theoretical basis for integrated management of wheat stripe rust and effective deployment of resistance genes in Pst over-summering zones.