Morphactin and 2iP markedly enhance accumulation of stilbenes in cell cultures of Cayratia trifolia (L.) Domin |
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Authors: | Chetana Roat and Kishan G Ramawat |
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Institution: | (1) Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, 313001, India |
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Abstract: | The cell cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in Murashige and Skoog’s medium containing 0.25 mg l−1 naphthalene acetic acid, 0.2 mg l−1 kinetin and 250 mg l−1 casein hydrolysate. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin) which on addition of 0.1–0.5 mg l−1 morphactin in the medium containing naphthalene acetic acid and kinetin declined. Morphactin or 2 isopentenyl adenine alone
at 0.1 mg l−1 concentration enhanced stilbenes which on combination markedly enhanced the yield to ~5 mg l−1 at 15th day. |
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Keywords: | Cayratia trifolia Morphactin Resveratrol Stilbenes Viniferin |
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