酿酒酵母表达系统

酿酒酵母是单细胞真核微生物,人们以酿酒酵母为宿主菌,用载体表达外源基因的过程中,积累了丰富的经验,掌握了酿酒酵母表达的许多优缺点,如它繁殖速度快,可以大规模发酵生产,但在发酵过程中会产生乙醇,而乙醇在培养基中积累会影响酵母的生长代谢和基因产物的表达,尤其是进行高密度发酵时该效应更明显;针对这些缺点,可以采取积极的应对措施.主要就酿酒酵母表达系统的组成、优缺点及高效表达的策略等作一综述.

Saccharomyces Serevisiae Expression System

Saccharomyces serevisiae is unicellular eukaryotic biology. People accumulate many experiences and grasp many advantages or disadvantages of yeast expression in the course of exogenous gene expression with vector in saccharomyces serevisiae, such as yeast grows speedily, and can be processed with massive fermentation. But it will also produce alcohol in the process of fermentation, and the accumulation of alcohol in the culture medium will affect yeast's growth metabolism and gene expression, this effect is especially obvious in the high density fermentation. Contraposing those defects, positive steps can be adopted. The composition, advantages and disadvantages,the strategies of effective expression of Saccharomyces serevisiae expression system are reviewed.