盐酸胍处理大麦类囊体膜对金属阳离子调节激发能分配的影响

以大麦（ＨｏｒｄｅｕｍＶｕｌｇａｒｅＬ）叶片为实验材料，研究盐酸胍处理大麦类囊体膜对金属阳离子调节激发能分配的影响。结果表明：当未用盐酸胍处理时，Ｍｇ２＋对类囊体膜的Ｆ６８６／Ｆ７３６比值有显著的刺激作用，而用不同浓度盐酸胍处理类囊体膜，则明显阻滞Ｍｇ２＋调节激发能向ＰＳＩＩ分配，表明盐酸胍处理可能损伤类囊膜上ＬＨＣ—ＩＩ与Ｍｇ２＋调节作用有关的蛋白质，从而导致Ｍｇ２＋调节效应降低．用ＳＤＳ—ＰＡＧＥ方法研究盐酸胍处理后的类囊体膜色素蛋白复合物的组成变化，结果表明盐酸胍处理导致ＭＩＣ—ＩＩ中ＭＩＣＰ１和ＬＨＣＰ２组分消失，这说明盐酸胍处理阻滞Ｍｇ２＋调节效应可能与ＬＪＩＣ—ＩＩ上的ＬＨＣＰ１，ＬＨＣＰ２受损有关。

EFFECT OF GUANIDINE HYDROCHLORIDE ON THE CATION-INDUCED EXCITATION ENERGY DISTRIBUTION IN THE BARLEY THYLAKID MEMBRANES

The results show that Mg2 induce a sigifciant increase of Chla F686/F736 at 77 K of thylakoid membranes without GuHCl. With GuHCl thylakoid membranes treated the Mg2 regulating excitation energy distribution effect was declined with the increase of the GuHCl concentration. The above evidence suppeds that GuHCl could damage proteins which is related to Mg2 -induced effects on LHC-II of thylakoid membranes. LHCP1 and LHCP2 components showed the disappearance of SDS-PAGE pattern of Chlorophyll-proteins complexes of the thylakoid memberanes treated with GuHCl. The results indicate that Mg2 -induced effects being lost on the thylkoid membranes modified by GuHCl may relate to the damage of LHCP1, LHCP2 components of LHC-II.