亲和素蛋白与含有生物素的支撑平面膜之间相互作用的研究

生物功能分子的二维有序化组装是分子工程学的重要内容，本文通过构建有生物素受体的人工模型膜，对亲和素与生物这一具有极强亲和力的系统之间的特异性相互作用的某些制约因素进行了探讨，应用ＬＢ膜技术结合激光椭圆偏振术和表面等离激元谱技术，较深入地研究了蛋白质与脂单层膜发发的非特异性吸附，特异性结合以及结合的侧向空间位阻效应，实验结果表明，膜表面电荷对非特异性吸附的速率有很大的影响，而对最终的蛋白质附量影响不

INTERACTION BETWEEN AVIDIN AND BIOTIN-CONTAINING SUPPORTED PLANAR MEMBRANE

Two-dimensional assmble of biological molecules is one important field in field in the molecular engineering. We.studied the interaction of avidin with phospholipid model membranes con taining biotin-conjugated phospholipids. Using LB technique together with sarface sensitive techniques (such as eilipsometry and sarface plasmon spectroscopy), we studied the nonspecific adsorption and specific binding of avidin to phospholipid lamella, and the effect of the lateral steric hindrance.The primary conclusions of the present work are as following first, the charges of membrane sarface effect the rate of protein adsorption, but do not effect the amount of protein ultimately adsorbed second, the nonspecific adsorption of avidin can be detached by adding a suitable amount of bivalent alkaline-earth metal ions; third, the binding of avidin to membrane bound model receptor is astricted remarkable by the latelal steric hindrence.