| 高山离子芥CbMAPK3基因克隆与原核表达 |
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| 引用本文： | 张腾国,刘玉冰,孙坤,杨宁,安黎哲. 高山离子芥CbMAPK3基因克隆与原核表达[J]. 植物研究, 2009, 29(6): 692-695 |
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| 作者姓名： | 张腾国 刘玉冰 孙坤 杨宁 安黎哲 |
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| 作者单位： | (1.西北师范大学生命科学院，兰州 730070) ;(2.中国科学院寒区旱区环境与工程研究所，兰州 730000) ;(3.兰州大学生命科学院，兰州 730000) |
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| 摘 要： | 促分裂原活化蛋白激酶（MAPK）在信号传导过程中发挥着重要的作用。以高山离子芥叶片为材料,利用RT-PCR法克隆到全长CbMAPK3基因cDNA，将其与大肠杆菌表达载体pET-30a连接，构建原核表达载体pET-30a-CbMAPK3，并转化大肠杆菌BL21,经IPTG诱导表达后,SDS-PAGE以及Western blot检测结果表明该基因表达了1个约46kD的蛋白,为进一步研究目的蛋白的结构和功能提供了实验基础。
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| 关 键 词： | 高山离子芥；CbMAPK3基因；原核表达 |
Cloning and Prokaryotic Expression of Chorispora bungeana CbMAPK3 Gene |
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| Affiliation: | (1.School of life science,Northwest Normal University,Lanzhou 730070) (2.Cold and Arid Regions Environmental and Engineering Institute,Chinese Academy of Sciences,Lanzhou 730000) (3.College of Life Science,Lanzhou University,Lanzhou 730070) |
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| Abstract: | Mitogen-activated protein kinase (MAPK) plays a central role in transfer information from diverse receptors/sensors to a wide range of cellular responses in plants. CbMAPK3 cDNA was amplified by RT-PCR from Chorispora bungeana leaf, then was cloned into pET-30a vector to construct recombinant prokaryotic expression vector pET-30a-CbMAPK3. The pET-30a-CbMAPK3 was transformed to E.coli strain of BL21. After induced by IPTG, a 46 kD recombinant protein was expressed and separated by SDS-PAGE electrophoresis and detected by western blot. The research provides a base for further studying on the protein structure and function of CbMAPK3. |
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| Keywords: | Chorispora bungeana CbMAPK3 gene prokaryotic expression |
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