Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro |
| |
Authors: | Yves Meyer Richard Cooke |
| |
Institution: | (1) Laboratoire de Physiologie Végétale, Université de Perpignan, F-66025 Perpignan-Cedex, France |
| |
Abstract: | The presence of auxin and cytokinin is necessary for the induction of mitosis in tobacco mesophyll protoplasts cultivated in vitro. In their absence, protoplasts firstly accumulate inhibitors of mitosis in the culture medium, possibly because of non-coordinated cell-wall synthesis, and secondly evolve a nonmitotic and degenerative metabolism. By changing the intoxified medium, it is possible to show that auxin is necessary from the beginning of culture, while cytokinin is only required later to allow a step in the development of the mitotic apparatus.Abbreviations 2.4-D
2.4 dichlorophenoxyacetic acid
- 6-BA
6-benzyladenine
- NAA
naphthaleneacetic acid
- IAA
indoleacetic acid |
| |
Keywords: | Mitosis Nicotiana Phytohormones Protoplasts |
本文献已被 SpringerLink 等数据库收录! |
|