Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis) |
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| Authors: | Katarina Cankar,Adè le van Houwelingen,Miriam Goedbloed,Rokus Renirie,René M. de Jong,Harro Bouwmeester,Dirk Bosch,Theo Sonke,Jules Beekwilder |
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| Affiliation: | 1. Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PD Wageningen, The Netherlands;2. Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands;3. Division of Molecular and Computational Toxicology, Free University of Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands;4. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;5. Isobionics, Urmonderbaan 22, 6167 RD Geleen, The Netherlands |
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| Abstract: | (+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 μg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases. |
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| Keywords: | CnVO, Callitropsis nootkatensis valencene oxidase CnVS, Callitropsis nootkatensis valencene synthase EST, expressed sequence tag |
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