The flanking sequence contributes to the immobilisation of spermine at the G-quadruplex in the NHE (nuclease hypersensitivity element) III1 of the c-Myc promoter |
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| Authors: | Max A. Keniry Elisabeth A. Owen |
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| Affiliation: | Research School of Chemistry, The Australian National University, Canberra, ACT 0200, Australia |
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| Abstract: | Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c-Myc promoter, nuclease hypersensitivity element III1 (NHE III1). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. |
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| Keywords: | bp, base-pair CD, circular dichroism NHE III1, nuclease hypersensitivity element III1 NMR, nuclear magnetic resonance NOE, Nuclear Overhauser Effect NOESY, Nuclear Overhauser Effect spectroscopy nt, nucleotide SCP, selective cross-polarisation TBA, thrombin-binding aptamer TOCSY, total correlation spectroscopy |
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