ANKHD1, a novel component of the Hippo signaling pathway,promotes YAP1 activation and cell cycle progression in prostate cancer cells |
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| Authors: | Joã o Agostinho Machado-Neto,Mariana Lazarini,Patricia Favaro,Gilberto Carlos Franchi Jr.,Alexandre Eduardo Nowill,Sara Teresinha Olalla Saad,Fabiola Traina |
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| Affiliation: | 1. Hematology and Hemotherapy Center, University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil;2. Department of Biological Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil;3. Integrated Center for Childhood Onco-Hematological Investigation, University of Campinas, Campinas, São Paulo, Brazil;4. Department of Internal Medicine, University of São Paulo at Ribeirão Preto Medical School, Ribeirão Preto, São Paulo, Brazil |
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| Abstract: | ANKHD1 is a multiple ankyrin repeat containing protein, recently identified as a novel member of the Hippo signaling pathway. The present study aimed to investigate the role of ANKHD1 in DU145 and LNCaP prostate cancer cells. ANKHD1 and YAP1 were found to be highly expressed in prostate cancer cells, and ANKHD1 silencing decreased cell growth, delayed cell cycle progression at the S phase, and reduced tumor xenograft growth. Moreover, ANKHD1 knockdown downregulated YAP1 expression and activation, and reduced the expression of CCNA2, a YAP1 target gene. These findings indicate that ANKHD1 is a positive regulator of YAP1 and promotes cell growth and cell cycle progression through Cyclin A upregulation. |
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| Keywords: | ANKHD1 YAP1 MASK Hippo pathway Prostate cancer Cell cycle |
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