Visualization of cell-substratum adhesion plaques by antibody exclusion |
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| Affiliation: | 1. Laboratório de Catálise e Materiais, Departamento de Química Geral e Inorgânica, Instituto de Química, Universidade Federal da Bahia, Trav. Barão de Jeremoabo, 147, Campus de Ondina, 40170-280 Salvador, BA, Brazil;2. Instituto Nacional de Ciência e Tecnologia em Energia e Ambiente (INCT – E&A), Centro Interdisciplinar de Energia e Ambiente (CIENAM), Campus de Ondina, 40170-115 Salvador, BA, Brazil |
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| Abstract: | ![]()
We report on an antibody exclusion method for detecting cell adhesion plaques. Substrata coated by plasma fibronectin (pFN) appeared uniformly fluorescent when analyzed by indirect immunofluorescence using a specific anti-pFN antibody. When cells that had spread on the substrata were permeabilized and analyzed by indirect immunofluorescence, areas of non-fluorescence were apparent beneath the cells and in focal streaks along the cell margins. The nonfluorescent focal streaks were coincident with adhesion plaques visualized by interference reflection microscopy. |
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