Calreticulin as a potential diagnostic biomarker for lung cancer |
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| Authors: | Rongrong Liu Jiuyu Gong Jun Chen Qi Li Chaojun Song Jian Zhang Yongming Li Zhijia Liu Yun Dong Lihua Chen Boquan Jin |
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| Institution: | Department of Immunology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an, China. |
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| Abstract: | Calreticulin (CRT) is an endoplasmic reticulum luminal Ca(2+)-binding chaperone protein. By immunizing mice with recombinant fragment (rCRT/39-272), six clones of monoclonal antibodies (mAbs) were generated and characterized. Based on these mAbs, a microplate chemiluminescent enzyme immunoassay (CLEIA) system with a measured limit of detection of 0.09?ng/ml was developed. Using this CLEIA system, it was found that soluble CRT (sCRT) level in serum samples from 58 lung cancer patients was significantly higher than that from 40 healthy individuals (only 9 were detectable, P?
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