硫化氢减轻同型半胱氨酸诱导大鼠海马CA1区神经元损伤

目的探讨硫化氢(H2S)对同型半胱氨酸(Hcy)诱导大鼠海马CA1区神经元损伤的改善作用。方法以侧脑室注射同型半胱氨酸的SD大鼠为同型半胱氨酸神经毒性动物模型,采用Tunel染色法分析大鼠海马CA1区神经元的凋亡情况,采用Elisa法分析大鼠海马组织MDA含量。结果 0.6μmol和2.0μmol的同型半胱氨酸使大鼠海马CA1区神经元发生大量的凋亡(P0.01),而NaHS(100μmol/kg)显著抑制同型半胱氨酸(0.6μmol)诱导大鼠海马CA1区神经元的凋亡(P0.05);同型半胱氨酸(0.2,0.6μmol)可增加大鼠海马CA1区丙二醛含量(P0.001),而NaHS(100μmol/kg)可抑制同型半胱氨酸(0.6μmol/L)诱导大鼠海马CA1区丙二醛水平的增加(P0.001)。结论硫化氢可减轻同型半胱氨酸诱导大鼠海马CA1区神经元的损伤。

Hydrogen Sulfide Attenuates Homocysteine-induced Neuronal Injury in the CA1 Area in Hippocampus of Rats

Objective This study was to explore whether Hydrogen sulfide ameliorates Homocysteine (Hcy)-induced neuronal damage in the CA1 area of hippocampus of rats.Methods This study used Sprague-Dawley (SD) rats intracerebroventricularly injected with Hcy as an animal model of Hcy-induced neurotoxicity.Neuronal apoptosis in hippocampus was analysed by Terminal-deoxynucleotidyl transferase-mediated dUTP nick-end labeling (Tunel) staining.Malondialdehyde (MDA) levels in the brains of rats were detected by Elisa.Results Hcy (0.6,2.0 μmol) induced neuronal apoptosis in the CA1 area in hippocampus of rats (P<0.01);NaHS (100 μmol/kg) markedly ameliorated neuronal apoptosis in hippocampus induced by Hcy (0.6 μmol) (P<0.05).Elisa results indicated that Hcy (0.2,0.6 μmol) increase the level of MDA in rat brain (P<0.001);the result of Elisa showed that NaHS (100 μmol/kg) significantly inhibited the increasing level of MDA induced by Hcy (0.6 μmol) (P<0.001).Conclusion H2S attenuates Hcy-induced neuronal injury in the CA1 area of hippocampus of rats.