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Optimization of Antimicrobial Production by a Marine Actinomycete Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India |
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| Authors: | R Vijayakumar K Panneerselvam C Muthukumar N Thajuddin A Panneerselvam R Saravanamuthu |
| Institution: | 1. Department of Microbiology, Bharathidasan University College (W), Orathanadu, 614 625, India 2. Department of Microbiology, Dr. G.R. Damodaran College of Science, Coimbatore, 641 014, India 3. Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia 4. Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, India 5. Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, 613 503, India 6. Department of Botany, A.V.C. College (Autonomous), Mannampandal, 609 305, India |
| Abstract: | Totally 25 marine soil samples were collected from the region of Palk Strait of Bay of Bengal, Tamil Nadu, and were subjected to the isolation of actinomycetes. Sixty-eight morphologically distinct isolates were obtained and 37% (25) of them had antimicrobial activity. The potential producer was named as Streptomyces sp. VPTS3-1 and the phylogenetic evaluation on the basis of 16S rDNA sequence further categorized the organism as Streptomyces afghaniensis VPTS3-1. Further, the antimicrobial compound was extracted from the isolate using various solvents and the antimicrobial efficacies were tested against bacterial and fungal pathogens. In addition, in vitro optimization of parameters for the antimicrobial compound production revealed that the suitable pH as 7–8, the period of incubation as 9 days, temperature (30°C), salinity (2%), and starch and KNO3 as the suitable carbon and nitrogen sources respectively in starch–casein medium. |
| Keywords: | Marine soils Streptomyces afghaniensis VPTS3-1 Antimicrobial activity Optimization |
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