Variation between mouse major urinary protein genes isolated from a single inbred line |
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| Authors: | A J Clark P M Clissold J O Bishop |
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| Affiliation: | Department of Genetics, University of Edinburgh, Edinburgh, EH9 3JN U.K. |
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| Abstract: | We describe ten Charon 4A genomic DNA clones from BALB/c mice which include at least seven different major urinary protein (MUP) genes. We have established the orientation of all seven sequences, and have placed six of them in precise register by means of restriction site maps and Southern blot hybridization with cloned cDNA sequences. Four of the seven genomic sequences (family I sequences) form hybrids with six independent cDNA clones that have a high thermal stability and hybridize more strongly with mRNA from three inbred mouse lines. Hybrids between the remaining three genomic sequences and the cDNA clones have a lower thermal stability and hybridize less strongly with mRNA from the three inbred lines. Homologies between different cloned sequences extend over as much as 15 kb. No clone contains parts of two MUP genes, and no homology has been detected between the 3' flanking region of one MUP gene and the 5' flanking region of another. |
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| Keywords: | Recombinant DNA cloned cDNA probes λ Charon vectors restriction site maps gene family cDNA complementary DNA EtBr ethidium bromide kb kilobase or kilobase pairs MUP major urinary protein SET 150 mM NaCl, 30 mM Tris·HCl, pH 8, 1 mM EDTA |
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