Analysis of the structure-functional features of a highly-repeating centromere sequence from the Fringilla coelebs L. (Aves: Passeriformes) genome] |
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Authors: | A G Malykh V G Zhurov A F Sa?fitdinova S E Deriusheva E R Gaginskaia |
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Abstract: | A highly repetitive centromeric Fringilla coelebs PstI (FCP) element was cloned and sequenced. The FCP tandem repeats with unit 505 or 506 nt accounted for about 0.9% of the entire genome and had 57% GC. Direct genomic sequencing with FCP-specific primers and ThermoFidelase 2A revealed the consensus sequence and the five most common single-nucleotide polymorphisms (SNPs) for the FCP unit. FCP may be transcribed and may play a role in spatial arrangement of the genome. |
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