| 高碘酸-硝酸银染色法鉴定糖蛋白 |
| |
| 引用本文： | 刘翠芳,蒋继志.高碘酸-硝酸银染色法鉴定糖蛋白[J].生物技术通报,2006(Z1):534-535. |
| |
| 作者姓名： | 刘翠芳 蒋继志 |
| |
| 作者单位： | 河北大学,生命科学学院,保定,071002 |
| |
| 基金项目： | 河北省自然科学基金;河北省省级重点学科生物工程项目 |
| |
| 摘 要： | 对传统的糖蛋白染色方法(高碘酸-Schiff法)进行了改进。蛋白质的氧化采用高碘酸法,染色时采用硝酸银染色法。此方法灵敏度是高碘酸-Schiff法的100倍,而且比高碘酸-Schiff法节省16~18h。
|
| 关 键 词： | 糖蛋白 聚丙烯酰胺凝胶电泳 银染 |
Silver Staining of Glycoproteins on a Polyarcrymide Gel |
| |
| Liu Cuifang,Jiang Jizhi.Silver Staining of Glycoproteins on a Polyarcrymide Gel[J].Biotechnology Bulletin,2006(Z1):534-535. |
| |
| Authors: | Liu Cuifang Jiang Jizhi |
| |
| Abstract: | The Staining of Glycoproteins on a Polyarcrymide Gel was developed by silver staining.Before the silver staining,the proteins were oxidized by Periodic acid.The sensitivity of silver staining was as high as 100 folds and the experimental time was shorten by 16~18hours in comparison with the Schiff staining |
| |
| Keywords: | Glycoproteins PAGE Silver staining |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
