| 迷走神经切除对大鼠胃内UCP2 mRNA表达及胃酸分泌的影响 |
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| 作者姓名： | Zou Y Yang M Gong DZ Guan LL Tian N |
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| 作者单位： | 1. 大连医科大学生理教研室,辽宁,大连,116027
2. 辽宁师范大学化学系,辽宁,大连,116029 |
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| 基金项目： | 教育部留学回国人员科研启动基金,辽宁省教育厅资助项目 |
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| 摘 要： | 目的:观察整体大鼠胃酸分泌与ATP水平之间的关系及迷走神经对解偶联蛋白2(UCP2)mRNA表达的调节.方法:制备大鼠高选择性迷走神经切断模型.采用滴定法检测大鼠胃酸酸度,荧光测定法检测胃体组织内ATP含量,应用Northern blot法检测分析UCP2mRNA表达.结果:迷走神经切断后24 h,大鼠胃酸酸度显著降低,胃体组织内ATP含量下降.与假手术组相比,胃体组织UCP2 mRNA表达显著增加.结论:迷走神经切断后24 h大鼠胃体组织ATP含量下降;迷走神经下调胃体组织UCP2 mRNA表达.结果提示大鼠迷走神经可能通过抑制UCP2 mRNA表达,提高ATP含量,为质子泵泌酸提供能量来源.
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| 关 键 词： | 解偶联蛋白2 胃迷走神经切断术 胃酸分泌 |
| 文章编号： | 1000-6834(2005)03-0290-03 |
| 修稿时间： | 2003年9月18日 |
Effects of vagotomy on UCP2 mRNA expression and gastric acid secretion in rats |
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| Zou Y,Yang M,Gong DZ,Guan LL,Tian N.Effects of vagotomy on UCP2 mRNA expression and gastric acid secretion in rats[J].Chinese Journal of Applied Physiology,2005,21(3):290-292. |
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| Authors: | Zou Yuan Yang Mei Gong De-Zheng Guan Li-Li Tian Nan |
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| Institution: | Department of Physiology, Dalian Medical University, Dalian 116027, China. |
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| Abstract: | Aim: The relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein2(UCP_2) mRNA expression by vagus nerve were studied in vagotomied rats. Methods: With the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluoremetry. The expression of UCP_2 mRNA was observed by using Northern blot in stomach of rats. Results: Both of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP_2 mRNA was markedly increased as compared with sham operation group. Conclusion: ATP contents decreased and vagus nerve down-regulates expression of UCP_2 mRNA in stomach corpus in vagotomied rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP_2 mRNA and increasing ATP contents in rats |
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| Keywords: | ATP |
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