Immunocytochemical Localization of (Na+,K+)-ATPase in the Goldfish Optic Nerve |
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Authors: | Michal Schwartz Stephen A Ernst George J Siegel Bernard W Agranoff |
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Institution: | Departments of Neurology, Anatomy, and Biological Chemistry, and Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan |
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Abstract: | Antiserum to the catalytic subunit of goldfish brain (Na+, K+)-ATPase has been employed at the electron microscopic level by means of the peroxidase-antiperoxidase immunohistochemical method. In optic nerve, antigenic sites are restricted to the nodes of Ranvier. No reaction product is detected in underlying internodal neurolemma. Outgrowing neurites for cultured retinal explants devoid of glial ensheathment exhibit a continuous distribution of the enzyme subunit. Antibodies against eel electroplax (Na+, K+)-ATPase cross-react with the goldfish brain enzyme and show a similar immunocytochemical distribution pattern. |
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Keywords: | (Na +K+) ATPase Immunocytochemistry Ultrastructure Goldfish visual system |
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