The possible role of metallothioneins in copper tolerance of Silene cucubalus |
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Authors: | P C Lolkema M H Donker A J Schouten W H O Ernst |
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Institution: | (1) Department of Ecology, Biological Laboratory, Free University, P.O. Box 7161, NL-1007 MC Amsterdam, The Netherlands |
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Abstract: | Growth and copper-binding of a copper-tolerant and a copper-sensitive population of Silene cucubalus (L.) Wib. have been studied. The copper-tolerant plants showed a much lower uptake and a proportionally higher transport of copper from root to shoot. A copper-binding protein with an apparent Mr of 8500 resembling metallothionein has been isolated from the roots of copper-treated plants of the tolerant population. After 20 d, the protein was observed to be inducible upon copper supply in the copper-tolerant plants, but not yet in the sensitive ones. This could be an indication of a difference in metalregulated synthesis of the protein. Ion-exchange chromatography of the 8500 protein yielded a major copper-containing fraction eluting at high ionic strength. Other characteristics such as UV absorption and amino-acid composition resembled strongly those of metallothioneins. The involvement of metallothioneins in the detoxification of copper within Cu-tolerant plants is discussed in relation to other mechanisms.Abbreviation DEAE
diethyloaminoethyl |
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Keywords: | Copper (uptake tolerance) Metallothionein Protein Cu-binding Silene Translocation (Cu) |
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