| SCP_3在小鼠精母细胞核内的表达及定位 |
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| 引用本文： | 郭睿,崔慧林,赵虹,张悦红,牛勃. SCP_3在小鼠精母细胞核内的表达及定位[J]. 中国组织化学与细胞化学杂志, 2008, 17(1): 84-88. DOI: 10.3870/j.issn.1004-1850.2008.01.018 |
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| 作者姓名： | 郭睿 崔慧林 赵虹 张悦红 牛勃 |
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| 作者单位： | 1. 山西医科大学组织学与胚胎学教研室,太原,030001
2. 山西医科大学生物化学与分子生物学教研室,太原,030001 |
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| 摘 要： | 目的探讨SCP，蛋白在雄性小鼠减数分裂各阶段精母细胞核内的表达及定位。方法采用免疫组织化学染色法初步检测SCP，蛋白在曲细精管中的细胞定位，进一步利用免疫荧光FITC／DAPI共染技术显示该蛋白在减数分裂各阶段精母细胞核内的表达。结果免疫组织化学染色结果显示，在完整的曲细精管内只有精母细胞呈阳性染色。免疫荧光分析证实在减数分裂1前期的细线期、偶线期、粗线期及双线期精母细胞核内均有明显的SCP，绿色荧光信号，并且在不同分裂阶段的细胞核内阳性着色条带的数目、状态不同。在处于终变期及中期Ⅰ的精母细胞核内，只有散在的片状或点状荧光信号，直至后期Ⅰ阶段完全消失。结论SCP，蛋白在雄性小鼠减数分裂各阶段精母细胞核内的表达与SC的形成、成熟、解体同步，参与了同源染色体之间的联会和重组。
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| 关 键 词： | 联会复合体 SCP3 减数分裂 同源染色体 小鼠 细胞核 表达 定位 MEIOSIS MOUSE DURING NUCLEUS LOCALIZATION 重组 同源染色体 同步 解体 完全 状态 色条 荧光信号 绿色 双线 粗线 |
| 修稿时间： | 2007-04-03 |
THE EXPRESSION AND LOCALIZATION OF SCP_3 IN THE NUCLEUS OF SPERMATOCYTES DURING MOUSE MEIOSIS |
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| Guo Rui,Cui Huilin,Zhao Hong,Zhang Yuehong,Niu Bo. THE EXPRESSION AND LOCALIZATION OF SCP_3 IN THE NUCLEUS OF SPERMATOCYTES DURING MOUSE MEIOSIS[J]. Chinese Journal of Histochemistry and Cytochemistry, 2008, 17(1): 84-88. DOI: 10.3870/j.issn.1004-1850.2008.01.018 |
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| Authors: | Guo Rui Cui Huilin Zhao Hong Zhang Yuehong Niu Bo |
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| Affiliation: | Guo Rui,Cui Huilin1,Zhao Hong,Zhang Yuehong,Niu Bo*(Department of Biochemistry , Molecular Biology,1Department of Histology , Embryology,Shanxi Medical University,Taiyuan 030001,China) |
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| Abstract: | Objective To investigate the expression and localization of SCP3 in the nucleus of spermatocytes at different stage of male mouse meiosis.Methods Immunohistochemistry was used to detect the preliminary cellular localization of SCP3 in the seminiferous tubules.Immunofluorescence staining with FITC and costaining with DAPI were used to demonstrate the localization of the protein in the nucleus at different stages of meiosis.Results Immunohistochemical staining showed that SCP3 was merely detected in the nucle... |
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| Keywords: | SC SCP3 Meiosis Homologous chromosomes |
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