Salivary Hsp72 does not track exercise stress and caffeine-stimulated plasma Hsp72 responses in humans |
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Authors: | Matthew B Fortes Martin Whitham |
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Institution: | (1) School of Sport, Health and Exercise Sciences, Bangor University, George Building, Holyhead Road, Bangor, Gwynedd, LL57 2PZ, UK;(2) Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Australia |
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Abstract: | Heat shock protein 72 (Hsp72) has been detected within saliva, and its presence may contribute to oral defence. It is currently
unknown how physiological stress affects salivary Hsp72 or if salivary Hsp72 concentrations reflect plasma Hsp72 concentrations.
We studied the effect of exercise upon salivary Hsp72 expression, and using caffeine administration, investigated the role
of sympathetic stimulation upon salivary Hsp72 expression. Six healthy males performed two treadmill running exercise bouts
in hot conditions (30°C) separated by 1 week in a randomized cross-over design, one with caffeine supplementation (CAF) the
other with placebo (PLA). Plasma and saliva samples were collected prior to, during and post-exercise and assayed for Hsp72
concentration by ELISA. Exercise significantly increased plasma Hsp72, but not salivary Hsp72 concentration. Mean salivary
Hsp72 concentration (5.1 ± 0.8 ng/ml) was significantly greater than plasma Hsp72 concentration (1.8 ± 0.1 ng/ml), and concentrations
of salivary and plasma Hsp72 were unrelated. Caffeine supplementation and exercise increased the concentration of catecholamines,
salivary α-amylase and total protein, whilst the salivary Hsp72:α-amylase ratio was lower in CAF. Salivary Hsp72 was not altered
by exercise stress nor caffeine supplementation, and concentrations did not track plasma Hsp72 concentration. |
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Keywords: | Adrenergic Catecholamines Cholinergic Heat shock proteins Saliva |
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