14‐3‐3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage |
| |
Authors: | Kousuke Kasahara Hidemasa Goto Masato Enomoto Yasuko Tomono Tohru Kiyono Masaki Inagaki |
| |
Institution: | 1. Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan;2. Department of Cellular Oncology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan;3. Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama, Okayama, Japan;4. Virology Division, National Cancer Center Research Institute, Chuo‐ku, Tokyo, Japan |
| |
Abstract: | 14‐3‐3 proteins control various cellular processes, including cell cycle progression and DNA damage checkpoint. At the DNA damage checkpoint, some subtypes of 14‐3‐3 (β and ζ isoforms in mammalian cells and Rad24 in fission yeast) bind to Ser345‐phosphorylated Chk1 and promote its nuclear retention. Here, we report that 14‐3‐3γ forms a complex with Chk1 phosphorylated at Ser296, but not at ATR sites (Ser317 and Ser345). Ser296 phosphorylation is catalysed by Chk1 itself after Chk1 phosphorylation by ATR, and then ATR sites are rapidly dephosphorylated on Ser296‐phosphorylated Chk1. Although Ser345 phosphorylation is observed at nuclear DNA damage foci, it occurs more diffusely in the nucleus. The replacement of endogenous Chk1 with Chk1 mutated at Ser296 to Ala induces premature mitotic entry after ultraviolet irradiation, suggesting the importance of Ser296 phosphorylation in the DNA damage response. Although Ser296 phosphorylation induces the only marginal change in Chk1 catalytic activity, 14‐3‐3γ mediates the interaction between Chk1 and Cdc25A. This ternary complex formation has an essential function in Cdc25A phosphorylation and degradation to block premature mitotic entry after DNA damage. |
| |
Keywords: | Cdc25 Chk1 DNA damage checkpoint phosphorylation 14‐3‐3 |
|
|