|
|||||
|
|
A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing |
|
| Authors: | Kaoru Umeda Yoshiyuki Seto Tomoko Kohda Masafumi Mukamoto Shunji Kozaki |
| Institution: | 1. Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8‐34 Tojo‐cho, Tennoji‐ku, Osaka 543‐0026;2. Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1‐58 Rinku‐Orai‐Kita, Izumisano‐shi, Osaka 598‐8531, Japan |
| Abstract: | A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986–1987 or 1999–2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism. |
| Keywords: | boNT/A gene cluster typing infant botulism multiplex PCR PFGE |