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Allelic Analyses of the Arabidopsis YUC1 Locus Reveal Residues and Domains Essential for the Functions of YUC Family of Flavin Monooxygenases |
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| Authors: | Xianhui Hou Sainan Liu Florencia Pierri Xinhua Dai Li-Jia Qu Yunde Zhao National Laboratory for Protein Engineering Plant Genetic Engineering Peking-Yale Joint Research Center for Plant Molecular Genetics AgroBiotechnology College of Life Sciences Peking University Beijing China Section of Cell Developmental Biology University of California at San Diego La Jolla California - USA |
| Institution: | Xianhui Hou1,2,Sainan Liu1,Florencia Pierri2,Xinhua Dai2,Li-Jia Qu1 and Yunde Zhao2 1National Laboratory for Protein Engineering and Plant Genetic Engineering,Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology,College of Life Sciences,Peking University,Beijing 100871,China 2Section of Cell and Developmental Biology,University of California at San Diego,La Jolla,California 92093-0116,USA |
| Abstract: | Flavin monooxygenases(FMOs) play critical roles in plant growth and development by synthesizing auxin and other signaling molecules.However,the structure and function relationship within plant FMOs is not understood.Here we defined the important residues and domains of the Arabidopsis YUC1 FMO,a key enzyme in auxin biosynthesis.We previously showed that simultaneous inactivation of YUC1 and its homologue YUC4 caused severe defects in vascular and floral development.We mutagenized the yuc4 mutant and screene... |
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