Glutamate Differently Modulates Metabotropic Glutamate Receptors in Neuronal and Glial Cells |
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Authors: | Carlos Alberto Castillo David Agustín León Inmaculada Ballesteros-Yáñez Inmaculada Iglesias Mairena Martín José Luis Albasanz |
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Institution: | 1.área de Bioquímica, Departamento de Química Inorgánica, Orgánica y Bioquímica, Facultad de Ciencias Químicas, Centro Regional de Investigaciones Biomédicas (CRIB),Universidad de Castilla-La Mancha (UCLM),Ciudad Real,Spain |
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Abstract: | Glutamate is an excitatory neurotransmitter implicated in learning and memory processes, but at high concentrations it acts
as an excitotoxin causing degeneration and neuronal death. The aim of this work was to determine the excitotoxic effect of
glutamate and the regulation of metabotropic glutamate receptors (mGluR) during excitotoxicity in neurons and C6 glioma cells.
Results show that glutamate causes excitotoxic damage only in cortical neurons. Loss of cell viability in neurons was glutamate
concentration- and time-dependent. Total mGluR levels were significantly reduced in these cells when exposed to glutamate.
However, in C6 cells, which have been used as a model of glial cells, these receptors were regulated in a biphasic manner,
decreased after 6 h, and increased after 24/48 h of treatment. Results show a cell dependent mGluR regulation by glutamate
exposure which could mediate the vulnerability or not to glutamate mediated excitotoxicity. |
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Keywords: | |
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