Assembly of the photosynthetic apparatus in embryos from Fucus serratus L |
| |
Authors: | Lamote Morgane Darko Eva Schoefs Benoît Lemoine Yves |
| |
Institution: | (1) Cytophysiologie Végétale et Phycologie, UPRESA-CNRS 8013, Université de Lille 1, 659655 Villeneuve d'Ascq, France;(2) Laboratoire d'Ecologie et Phycologie, Université, Laval, Québec, G1K 7P4, Canada;(3) Ecole Normale Supérieure, CNRS – UMR 8543, Dynamique des Membranes Végétales, Complexes Protéines-Pigments, 46 rue d'Ulm, 75230 Paris Cedex 5, France;(4) Present address: Phytobiologie Cellulaire, UMR-INRA/UB1088 – FRE-CNRS 2625 – Plante-Microbe-Environnement, Université de Bourgogne à Dijon, BP 47870, 21078 Dijon Cedex, France |
| |
Abstract: | The assembly of the photosynthetic apparatus was studied during the first six days of development of Fucus serratus L. embryos. HPLC analysis revealed that oospheres and zygotes contain the same photosynthetic pigments (i.e., chlorophyll
a, chlorophyll c, fucoxanthin, violaxanthin, and β-carotene) as fully developed thalli. Total pigment amount increased after fertilization,
mainly due to an active synthesis of Chl a and fucoxanthin. Spectral modifications revealing the progressive integration of Chl a and Chl c in the photosynthetic units are described. In particular, a distinct emission at 705 nm, reflecting the accumulation of LHC
I, was clearly detected. The emission bands at 705 nm and 725 nm were characterized by 77 K excitation fluorescence measurements.
Their spectra differed by the presence of a large band at approximately 550 nm due to fucoxanthin in the excitation spectrum
of F705 nm. Room temperature variable fluorescence was first observed 30 h after fertilization indicating a functional Photosystem
II electron transfer at this developmental stage.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | Biogenesis of the photosynthetic apparatus embryonic tissues macrophytes photosynthesis pigment– protein complexes seaweeds steady-state fluorescence spectroscopy variable fluorescence |
本文献已被 PubMed SpringerLink 等数据库收录! |
|