Osteoblast cytoskeletal modulation in response to compressive stress at physiological levels |
| |
Authors: | Juan Li Guoping Chen Leilei Zheng Songjiao Luo Zhihe Zhao |
| |
Institution: | (1) Division of Rheumatology, Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Firat University, Firat Tip Merkezi, FTR AD, Romatoloji BD, 23119 Elazig, Turkey;(2) Department of Physiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey;(3) Department of Physiology, Faculty of Medicine, Inonu University, Malatya, Turkey |
| |
Abstract: | In this study we assessed activities of antioxidant enzymes, lipid peroxidation end-products, and nitric oxide (NO) levels
in women with postmenopausal osteoporosis (PMO). Relationship between oxidative stress parameters and NO levels with bone
mineral density (BMD) and clinical variables influencing bone mass and health related quality of life measures was also investigated
in women with PMO.
Postmenopausal women (n = 87), aged 40–65, without previous diagnosis or treatment for osteoporosis and independent in daily living activities were
included. BMD was measured at the lumbar spine and proximal femur using dual-X-ray absorptiometry (DXA). Erythrocyte catalase
(CATe) enzyme activity, erythrocyte and plasma enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px),
and lipid peroxidation end-product malondialdehyde (MDA) and nitrite/nitrate levels, by product of NO were studied. A total
of 23 healthy non-porotic women were included as controls.
Women with PMO had significantly lower erythrocyte CATe enzyme activity and higher erythrocyte malondialdehyde (MDAe) and
erythrocyte nitric oxide (NOe) levels in comparison to controls whereas erythrocyte SODe and GSH-Px enzyme activity was similar.
In plasma, osteporotic women had significantly higher SOD enzyme activity and higher MDA levels whereas similar GSH-Px enzyme
activity and NO levels compared to non-porotic controls. Significant correlation was found between erythrocyte SODe, CATe
enzyme activity and NOe levels with proximal femur BMD. Some of the quality of life scores as pain, mental, and social functions
correlated with antioxidant enzyme activities and NO levels.
Consequently, oxidative stress markers may be an important indicator for bone loss in postmenopausal women. Further researches
assessing the oxidative stress markers and NO in bone tissue and changes with anti-osteoporotic drugs would be valuable to
better understand the role of free radicals, antioxidants, and NO in the regulation of bone mass. |
| |
Keywords: | antioxidant enzyme malondialdehyde nitric oxide postmenopausal osteoporosis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|