Osteogenic and osteoclastic cell interaction: development of a co-culture system |
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| Authors: | P M Loomer Richard P Ellen Howard C Tenenbaum |
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| Institution: | School of Dentistry, Department of Stomatology, Division of Periodontology, University of California, San Francisco, 521 Parnassus Avenue, Room C-628, San Francisco, CA 94143-0650, USA Tel.: +1 415 476 1458; Fax: +1 415 502 4990, US
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada, CA
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| Abstract: | The processes involved in the regulation of bone cell metabolism are complex, including those implicated in bone cell coupling. This study was undertaken to develop a model that would permit real-time interaction between osteoclastic cells and osteoblasts in vitro. Osteogenic bone marrow stromal cells were isolated from 18-day-old embryonic chickens, while osteoclastic cells were isolated from laying White Leghorn hens on calcium-deficient diets. Osteoclastic cells (5×105) were seeded onto mineral thin films and suspended above osteogenic cells (1×104) already plated on the bottoms of tissue culture plate wells. The data showed that after 4 days of incubation there was up to a fivefold (P<0.05) reduction in all measured parameters of osteogenesis (mineralization, alkaline phosphatase activity and type I collagen production) in osteogenic cultures grown in the presence of osteoclastic cells. Similarly, osteoclastic cell-induced mineral resorption was reduced up to threefold (P<0.05). Co-culture effects on cellular responses could be manipulated by known antiresorptive agents (e.g., pamidronate) altering either the source or the age of osteoclastic cells. The results indicate that the co-culture model may be useful in the study of bone cell interactions. |
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