Pathogenicity and life cycle of Meloidogyne javanica on broccoli

A pathogenicity trial conducted against root-knot nematode, Meloidogyne javanica on broccoli indicated that a gradual increase in the nematode inoculum from 500 to 8000 juveniles/kg soil was associated with a progressive decline in all the plant growth parameters and reproduction factor of the nematode. Although 8000 juveniles/kg soil showed maximum plant growth reduction and root knot index, statistical analysis of the data revealed that the population of 1000 juveniles/kg soil was associated with a significant decline in plant growth. Hence, this level was indicative of being the pathogenic level. The significant reduction in seedling emergence was recorded at and above 2000 juveniles/kg soil and it decreased further with increasing inoculum levels. Meloidogyne javanica required 27 days to complete the life cycle on broccoli at a temperature range of 28–35°C.