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Investigation on the Fusarium induced stress protein (FISP) in wheat: Immunolocalisation in root cells |
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| Authors: | B Mittra A B Das R K Behera A Mohammad T K Das P Mohanty |
| Institution: | 1. School of Biotechnology, Fakir Mohan University , Vyasa Vihar, Balasore, 756019, Orissa, India bhaba_mit@sify.com;3. Cytogenetics Laboratory, Regional Plant Resource Centre , Nayapalli, Bhubaneswar, Orissa, India;4. School of Biotechnology, Fakir Mohan University , Vyasa Vihar, Balasore, 756019, Orissa, India;5. Department of Biology, Life Science Building , McMaster University , Ontario, Canada;6. Electron Microscopic Facility , All India Institute of Medical Sciences , New Delhi, 110019, India;7. Hyderabad Central University , Hyderabad, India |
| Abstract: | Abstract Fusarium induced-stress-protein (FISP) of ～51 kDa molecular mass was detected in seven day old germinated wheat (Triticum aestivum var Sonalika) seedlings infected with F. oxysporum for a period of seven days. This particular stress protein (FISP) of ～51 kDa was over-expressed in the case of Fusarium infected seedlings compared to the untreated seedlings where the presence of this protein was insignificant. Localisation of this ～51 kDa protein in root tissue by anti-CSAP (Cadmium Stress Associated Protein) antiserum showed a significantly higher number of gold particles in the case of Fusarium infected root tissue compared to the untreated control. A unique type of organised localisation of FISP around the plasma membrane and outer vacuolar membrane suggests its defensive role against Fusarium infection that might be a general stress protein against biotic and abiotic stresses. |
| Keywords: | Triticum Fusarium Fusarium-induced-stress-protein immunolocalisation |