Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon |
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| Authors: | Kouhei Kishida Natsumi Ogawa Eikichi Ichihashi Hiromi Kato Yuji Nagata Yoshiyuki Ohtsubo |
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| Institution: | Graduate School of Life Sciences, Tohoku University , Sendai, Japan |
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| Abstract: | Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene. |
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| Keywords: | Mycobacterium vector plasmid gene disruption PAH microbial degradation |
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