Laboratory acetylene reduction assay for relative measurement of N2-fixing activities associated with field-grown wetland rice plants |
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| Authors: | Barraquio W. L. Daroy M. L. G. Tirol A. C. Ladha J. K. Watanabe I. |
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| Affiliation: | (1) Department of Soil Microbiology, The International Rice Research Institute, Los Baños, Laguna, Philippines;(2) Present address: Department of Microbiology, MacDonald Campus of McGill University, H9X 1CO Ste. Anne de Bellevue, Quebec, Canada |
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| Abstract: | Summary A short-term laboratory acetylene reduction assay using cut plant-soil samples incubated in the dark was developed for measuring relative N2-fixing activities associated with field-grown rice plants. The assay sample consists of rhizosphere soil, root, and cut stem and leaf sheath. The cut plant-soil assay is relatively simple, rapid, and convenient; it reduces, if not eliminates, the problems encountered in whole-plant (field, pot, and water culture) and excised roots assays. Varietal differences in N2-fixing activity were detected with the new assay technique. |
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| Keywords: | Acetylene reduction Associative N2-fixation Cut plant-soil Maahas clay Wetland rice |
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