Novel SNPs in the caprine stearoyl-CoA desaturase (SCD) and decorin (DCN) genes that are associated with growth traits in Chinese goat breeds |
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Authors: | Zhongqi Chen Jiajie Sun Zhuanjian Li Xianyong Lan Chunlei Zhang Yujiao Qu Yanli Liu Xingtang Fang Chuzhao Lei Hong Chen |
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Institution: | (1) College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No.22 Xinong Road, Yangling, 712100, Shaanxi, China;(2) Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, 221116, Jiangsu, China |
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Abstract: | Stearoyl-CoA desaturase (SCD) is an iron-containing enzyme involving in the biosynthesis of monounsaturated fatty acids (MUFA)
in mammary gland and adipose tissue, while decorin (DCN) consists of a protein core and a single dermatan or chondroitin sulfate
glycosaminoglycan chain, contributing multifunctionally to matrix assembly, modulation of the activity of growth factors and
cell migration and proliferation. However, few studies have focused on the genetic variability of them in goat. Herein, five
Chinese goat breeds (1229 animals) were analyzed. Based on DNA pooling and PCR-RFLP, three nucleotide substitutions, one of
which caused a amino acid substitution, were detected in SCD gene and three haploids (A, B, C) were constructed. According to SSCP analysis and DNA sequencing methods, a 2-bp deletion
and two other SNPs were found existing in another analyzed gene DCN, and three haploids (X, Y, Z) were built. Associations between the genotypes and the growth traits (body length, body height,
chest circumference, cannon circumference) were also analyzed. For SCD gene, genotype CC individuals had significant greater
body height in Guanzhong and body length in both Guanzhong and Xinong saanen than genotype BC individuals (P < 0.05). For DCN gene, individuals with genotype XX was obviously higher than that with genotype XY (P < 0.05). These results indicated that genotype CC of SCD gene and genotype XX of DCN gene could be used for the breeding of new breeds of goat in China. |
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